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Science. 2016 May 13;352(6287):816-8. doi: 10.1126/science.aaf6116. Epub 2016 Apr 10.

Zika virus impairs growth in human neurospheres and brain organoids.

Author information

1
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil.
2
D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil.
3
Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
4
D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil. Institute of Biology, State University of Campinas, Campinas, Brazil.
5
D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil. Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

Abstract

Since the emergence of Zika virus (ZIKV), reports of microcephaly have increased considerably in Brazil; however, causality between the viral epidemic and malformations in fetal brains needs further confirmation. We examined the effects of ZIKV infection in human neural stem cells growing as neurospheres and brain organoids. Using immunocytochemistry and electron microscopy, we showed that ZIKV targets human brain cells, reducing their viability and growth as neurospheres and brain organoids. These results suggest that ZIKV abrogates neurogenesis during human brain development.

PMID:
27064148
DOI:
10.1126/science.aaf6116
[Indexed for MEDLINE]
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