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Curr Protoc Neurosci. 2016 Apr 8;75:6.29.1-6.29.19. doi: 10.1002/cpns.8.

Electrophysiological Measurement of Cannabinoid-Mediated Synaptic Modulation in Acute Mouse Brain Slices.

Author information

1
Department of Psychiatry, Vanderbilt University Medical Center, Nashville, Tennessee.
2
Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, Tennessee.
3
Department of Molecular Physiology & Biophysics, Vanderbilt University Medical Center, Nashville, Tennessee.

Abstract

Endocannabinoids (eCBs) are a class of bioactive lipids that mediate retrograde synaptic modulation at central and peripheral synapses. The highly lipophilic nature of eCBs and the pharmacological tools available to interrogate this system require unique methodological consideration, especially when applied to ex vivo systems such as electrophysiological analysis in acute brain slices. This unit provides protocols for measuring cannabinoid and eCB-mediated synaptic signaling in mouse brain slices, including analysis of short-term, long-term, and tonic eCB signaling modes, and the unique considerations for working with eCBs and TRPV1/cannabinoid ligands in acute brain slices.

KEYWORDS:

CB1 receptor; cannabinoid; electrophysiology; endocannabinoid; mouse

PMID:
27063786
PMCID:
PMC4866814
DOI:
10.1002/cpns.8
[Indexed for MEDLINE]
Free PMC Article

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