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Eur J Immunol. 2016 Jun;46(6):1529-32. doi: 10.1002/eji.201546108. Epub 2016 Apr 24.

Neurons exhibit Lyz2 promoter activity in vivo: Implications for using LysM-Cre mice in myeloid cell research.

Author information

1
Carl-Ludwig Institute of Physiology, Leipzig University, Leipzig, Germany.
2
Institute of Anatomy, Leipzig University, Leipzig, Germany.
3
Institute of Biochemistry, Leipzig University, Leipzig, Germany.

Abstract

To characterize LysM-Cre mediated gene targeting in mice, we crossed LysM-Cre mice to two independent reporter-mouse lines (tdTomato or YFP). Surprisingly, we found that more than 90% of cells with LysM-Cre mediated recombination in the brain were neurons, rather than myeloid cells, such as microglia. Hence, by using the LysM-Cre mouse line for conditional knockout approaches, a significant neuronal recombination needs to be considered.

KEYWORDS:

Brain; LysM-Cre; Lyz2; Microglia; Neuroinflammation; Neurons

PMID:
27062494
DOI:
10.1002/eji.201546108
[Indexed for MEDLINE]
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