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Ticks Tick Borne Dis. 2016 Jul;7(5):817-827. doi: 10.1016/j.ttbdis.2016.03.017. Epub 2016 Mar 31.

Amblyomma parvum Aragão, 1908 (Acari: Ixodidae): Phylogeography and systematic considerations.

Author information

1
Institute for Coastal Plain Science, Georgia Southern University, Statesboro, GA 30460, USA. Electronic address: pl00967@georgiasouthern.edu.
2
Instituto Nacional de Tecnologia Agropecuaria, Estación Experimental Agropecuaria Rafaela, Santa Fe, Argentina.
3
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
4
Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil.
5
Department of Biology, Georgia Southern University, P.O. 8042, Statesboro, GA 30460, USA.
6
Departamento de Investigación en Entomología Médica, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panama, Panama.
7
Dipartamento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, Milano, Italy.
8
Escuela de Biología, Universidad de Costa Rica, San José, Costa Rica.
9
Institute for Coastal Plain Science, Georgia Southern University, Statesboro, GA 30460, USA.

Abstract

The geographical distribution of Amblyomma parvum Aragão 1908 in the New World is disjunct, with two main clusters separated from each other by the Amazon basin. The main objectives of this study were to further investigate the systematic relationships within A. parvum, to determine whether or not populations from different geographical areas might represent cryptic species, and to reconstruct the phylogeographical evolutionary history of the species. The genetic diversity of A. parvum collected throughout its distributional range was analyzed by using 6 molecular markers: 5 mitochondrial [the small and the large ribosomal subunits 12rDNA and 16SrDNA, the cytochrome oxidase I (COI) and II (COII) and the control region or d-loop (DL)], and one nuclear (ITS2, Inter transcribed spacer 2). Phylogenetic trees were inferred by using maximum parsimony and Bayesian analyses. In addition, node dating was attempted for the main lineages identified phylogenetically. Although mitochondrial and nuclear topologies were not totally congruent, they all identified at least two main supported clusters, a Central American lineage, and a Brazilian-Argentinian lineage. Clade support and divergence values strongly suggest that the two lineages correspond to different taxonomic entities. Node dating placed the split between the Central American and the Brazilian-Argentinian lineages at approximately 5.8-4.9 Mya, just after the progressive replacement of the dry areas that occupied the northern part of South America by the Amazon Basin in the early-mid Miocene. This event might be the cause of fragmentation and putative speciation within the ancestral relatively xerophilic A. parvum population.

KEYWORDS:

Amblyomma parvum; Cryptic species; Molecular markers; Phylogeny; Phylogeography; Systematics

PMID:
27062445
DOI:
10.1016/j.ttbdis.2016.03.017
[Indexed for MEDLINE]

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