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Cryobiology. 2016 Jun;72(3):239-43. doi: 10.1016/j.cryobiol.2016.03.012. Epub 2016 Apr 4.

Supercooling, ice nucleation and crystal growth: a systematic study in plant samples.

Author information

1
Department of Forestry and Management of Natural Environment, Technological Educational Institute (TEI) of Thessaly, 413 35 Larissa, Greece.
2
Department of Computer Engineering, Technological Educational Institute (TEI) of Thessaly, 413 35 Larissa, Greece.
3
Department of Plant Production, Technological Educational Institute (TEI) of Thessaly, 413 35 Larissa, Greece. Electronic address: anastaso@teilar.gr.

Abstract

This paper presents an innovative technological platform which is based on infrared video recording and is used for monitoring multiple ice nucleation events and their interactions, as they happen in 96 well microplates. Thousands of freezing curves were obtained during this study and the following freezing parameters were measured: cooling rate, nucleation point, freezing point, solidus point, degree of supercooling, duration of dendritic phase and duration of crystal growth. We demonstrate the use of this platform in the detection of ice nuclei in plant samples. Future applications of this platform may include breeding for frost tolerance, cryopreservation, frozen food technology and atmospheric sciences.

KEYWORDS:

Freezing; Grape; High throughput screening; Ice nucleation; Latent heat; Sea buckthorn; Supercooling; Thermal image processing

PMID:
27056262
DOI:
10.1016/j.cryobiol.2016.03.012
[Indexed for MEDLINE]

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