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Cell Mol Neurobiol. 2017 Mar;37(2):251-261. doi: 10.1007/s10571-016-0365-0. Epub 2016 Apr 6.

Hck Promotes Neuronal Apoptosis Following Intracerebral Hemorrhage.

Author information

1
Department of Geriatric Medicine, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
2
Department of Pathogen Biology, Medical College, Nantong University, Nantong, 226001, Jiangsu, China.
3
Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
4
Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu, China. ntdxtaotao@163.com.
5
Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China. shijiansheng2016@163.com.

Abstract

The hematopoietic cell kinase (Hck) is a member of the Src family protein kinases which regulates many signal transduction pathways including cell growth, proliferation, differentiation, migration, and apoptosis. However, the expression and function of Hck after intracerebral hemorrhage (ICH) are unknown. Western blot, immunohistochemistry, and immunofluorescence showed that Hck was obviously up-regulation in neurons adjacent to the hematoma after ICH. In addition, the temporary raise of Hck expression was paralleled with the expression of p53, Bax, and active caspase-3, suggesting that Hck was involved in neuronal apoptosis. Hck siRNA dramatically decrease hemin-induced expression of p53, Bax, and active caspase-3 as well as the amount of apoptotic SH-SY5Y cells in vitro. Furthermore, Hck interacted with p53. Hence, Hck might promote neuronal apoptosis via p53 signaling pathway after ICH.

KEYWORDS:

Hck; Intracerebral hemorrhage; Neuronal apoptosis

PMID:
27053350
DOI:
10.1007/s10571-016-0365-0
[Indexed for MEDLINE]

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