Format

Send to

Choose Destination
Methods Mol Biol. 2016;1502:159-68. doi: 10.1007/7651_2016_340.

A Microfluidic Bioreactor for Toxicity Testing of Stem Cell Derived 3D Cardiac Bodies.

Author information

1
Division of Biotechnology, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, 58183, Sweden.
2
Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, Carl-Neuberg-Str. 1, Hannover, 30625, Germany.
3
Division of Biotechnology, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, 58183, Sweden. cfm@ifm.liu.se.

Abstract

Modeling tissues and organs using conventional 2D cell cultures is problematic as the cells rapidly lose their in vivo phenotype. In microfluidic bioreactors the cells reside in microstructures that are continuously perfused with cell culture medium to provide a dynamic environment mimicking the cells natural habitat. These micro scale bioreactors are sometimes referred to as organs-on-chips and are developed in order to improve and extend cell culture experiments. Here, we describe the two manufacturing techniques photolithography and soft lithography that are used in order to easily produce microfluidic bioreactors. The use of these bioreactors is exemplified by a toxicity assessment on 3D clustered human pluripotent stem cells (hPSC)-derived cardiomyocytes by beating frequency imaging.

KEYWORDS:

3D cell culture models; Cardiac bodies (CBs); Cardiomyocytes; Drug assessment; Human pluripotent stem cells (hPSCs); Microfluidics; Organ-on-a-chip; Photolithography; Soft lithography

PMID:
27052611
DOI:
10.1007/7651_2016_340
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center