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Ren Fail. 2016 Jul;38(6):1007-20. doi: 10.3109/0886022X.2016.1163998. Epub 2016 Apr 6.

Ameliorative effect of naringin in acetaminophen-induced hepatic and renal toxicity in laboratory rats: role of FXR and KIM-1.

Author information

1
a Department of Pharmacology, Poona College of Pharmacy , Bharati Vidyapeeth Deemed University , Pune , Maharashtra , India.

Abstract

CONTEXT:

Acetaminophen (APAP) is an analgesic and antipyretic agent commonly known agent to cause hepatic and renal toxicity at a higher dose. Naringin, a bioflavonoid possesses multiple pharmacological properties such as antioxidant, anti-inflammatory, analgesic and anti-hyperlipidemic activity.

OBJECTIVE:

To evaluate the effect of naringin against the APAP-induced hepatic and renal toxicity.

MATERIALS AND METHODS:

Male Wistar albino rats (180-220 g) were divided into various groups, and toxicity was induced by APAP (700 mg/kg, p.o., 14 days). Naringin (20, 40 and 80 mg/kg, p.o.) or Silymarin (25 mg/kg) was administered to rats 2 h before APAP oral administration. Various biochemical, molecular and histopathological parameter were accessed in hepatic and renal tissue.

RESULTS:

Naringin pretreatment significantly decreased (p < 0.05) serum creatinine, blood urea nitrogen, bilirubin, aspartate transaminase, alanine transaminase, lactate dehydrogenase, low-density lipoprotein, very low-density lipoprotein, cholesterol and triglycerides as compared with APAP control rats. Decreased level of serum albumin, uric acid, and high-density lipoprotein were also significantly restored (p < 0.05) by naringin pretreatment. It also significantly restores (p < 0.05) the altered level of superoxide dismutase, reduced glutathione, malondialdehyde and nitric oxide in hepatic and renal tissue. Moreover, altered mRNA expression of hepatic farnesoid X receptor and renal injury molecule-1 (KIM-1) were significantly restored (p < 0.05) by naringin treatment. Naringin treatment also reduced histological alteration induced by APAP in the liver and kidney.

CONCLUSION:

Naringin exerts its hepato- and nephroprotective effect via modulation of oxido-nitrosative stress, FXR and KIM-1 mRNA expression.

KEYWORDS:

Antioxidant; FXR; KIM-1; hepatic toxicity; naringin; renal toxicity

PMID:
27050864
DOI:
10.3109/0886022X.2016.1163998
[Indexed for MEDLINE]

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