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J Ethnopharmacol. 2016 Jun 20;186:125-135. doi: 10.1016/j.jep.2016.03.068. Epub 2016 Apr 2.

Astragalus polysaccharides decrease muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling in 5/6 nephrectomised rats.

Author information

1
Department of Traditional Chinese Medicine, ZhuJiang Hospital, Southern Medical University, Guangzhou 510280, China; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China; Department of Nephrology, Southern Medical University TCM-Integrated Hospital, Guangzhou 510515, China.
2
Department of Traditional Chinese Medicine, the First People's Hospital of Shunde Affiliated to Southern Medical University, Guangzhou 528300, China.
3
Department of Traditional Chinese Medicine, ZhuJiang Hospital, Southern Medical University, Guangzhou 510280, China.
4
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
5
Department of Endocrinology, The Third Affiliated Hospital, Southern Medical University, Guangzhou 510280, China.
6
Department of Traditional Chinese Medicine, ZhuJiang Hospital, Southern Medical University, Guangzhou 510280, China; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China; Department of Nephrology, Southern Medical University TCM-Integrated Hospital, Guangzhou 510515, China. Electronic address: weilianbo@163.com.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Existing evidences suggest that Radix Astragali and its polysaccharides composition (APS) can improve muscle mass, but the mechanisms need more research.

AIM OF THE STUDY:

In this study, we aimed to examine the effects of APS on muscle wasting at molecular level in 5/6 nephrectomised rats.

MATERIALS AND METHODS:

We performed 5/6 nephrectomy or sham operation in 160 6-week-old Sprague-Dawley rats, and feed animals with or without 2% APS for 155 days. After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group. In addition, we detected the Akt/mTOR, ubiquitin proteasome, autophagy signalling and AA transporters in vivo and in vitro.

RESULTS:

Data in vivo show 2% APS could alleviate weight loss and improve protein metabolism in nephrectomised rats. The levels of serum pro-inflammatory factors and oxidative factors were restored by APS treatment. In molecular levels, APS restored Akt/mTOR, MAFbx, MuRF1, Atg7, LC3B-II/LC3B-I and SLC38A2 which changed in nephrectomised rats. Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy.

CONCLUSIONS:

Our results suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling, and SLC38A2 may be one of potential targets.

KEYWORDS:

Astragalus polysaccharides; Chronic kidney disease; Muscle wasting; Protein metabolism

PMID:
27049295
DOI:
10.1016/j.jep.2016.03.068
[Indexed for MEDLINE]

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