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J Cell Biol. 2016 Apr 11;213(1):23-32. doi: 10.1083/jcb.201512029. Epub 2016 Apr 4.

Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton.

Author information

1
Department of Biological Sciences, Dartmouth College, Hanover, NH 03755 The Bell Center, Marine Biological Laboratory, Woods Hole, MA 02543.
2
Department of Biological Sciences, Dartmouth College, Hanover, NH 03755.
3
Department of Physics, Institute for Biophysical Dynamics and James Franck Institute, University of Chicago, Chicago, IL 60637.
4
Department of Biological Sciences, Dartmouth College, Hanover, NH 03755 The Bell Center, Marine Biological Laboratory, Woods Hole, MA 02543 amy.gladfelter@dartmouth.edu.

Abstract

Cells change shape in response to diverse environmental and developmental conditions, creating topologies with micron-scale features. Although individual proteins can sense nanometer-scale membrane curvature, it is unclear if a cell could also use nanometer-scale components to sense micron-scale contours, such as the cytokinetic furrow and base of neuronal branches. Septins are filament-forming proteins that serve as signaling platforms and are frequently associated with areas of the plasma membrane where there is micron-scale curvature, including the cytokinetic furrow and the base of cell protrusions. We report here that fungal and human septins are able to distinguish between different degrees of micron-scale curvature in cells. By preparing supported lipid bilayers on beads of different curvature, we reconstitute and measure the intrinsic septin curvature preference. We conclude that micron-scale curvature recognition is a fundamental property of the septin cytoskeleton that provides the cell with a mechanism to know its local shape.

PMID:
27044896
PMCID:
PMC4828694
DOI:
10.1083/jcb.201512029
[Indexed for MEDLINE]
Free PMC Article

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