Format

Send to

Choose Destination
Stem Cell Res Ther. 2016 Apr 4;7:49. doi: 10.1186/s13287-016-0297-0.

VCAM-1+ placenta chorionic villi-derived mesenchymal stem cells display potent pro-angiogenic activity.

Author information

1
The State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, No.288, Nanjing Road, Heping District, Tianjin, 300020, China.
2
Beijing Institute of Health and Stem Cells, No.1 Kangding Road, BDA, Beijing, 100176, China.
3
National Engineering Research Center of Cell Products, No.80, Fourth Avenue, TEDA, Tianjin, 300457, China.
4
The State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, No.288, Nanjing Road, Heping District, Tianjin, 300020, China. zhibohan@163.com.
5
The State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, No.288, Nanjing Road, Heping District, Tianjin, 300020, China. hanzhongchao@hotmail.com.
6
Beijing Institute of Health and Stem Cells, No.1 Kangding Road, BDA, Beijing, 100176, China. hanzhongchao@hotmail.com.
7
National Engineering Research Center of Cell Products, No.80, Fourth Avenue, TEDA, Tianjin, 300457, China. hanzhongchao@hotmail.com.

Abstract

INTRODUCTION:

Mesenchymal stem cells (MSCs) represent a heterogeneous cell population that is promising for regenerative medicine. The present study was designed to assess whether VCAM-1 can be used as a marker of MSC subpopulation with superior angiogenic potential.

METHODS:

MSCs were isolated from placenta chorionic villi (CV). The VCAM-1(+/-) CV-MSCs population were separated by Flow Cytometry and subjected to a comparative analysis for their angiogenic properties including angiogenic genes expression, vasculo-angiogenic abilities on Matrigel in vitro and in vivo, angiogenic paracrine activities, cytokine array, and therapeutic angiogenesis in vascular ischemic diseases.

RESULTS:

Angiogenic genes, including HGF, ANG, IL8, IL6, VEGF-A, TGFβ, MMP2 and bFGF, were up-regulated in VCAM-1(+)CV-MSCs. Consistently, angiogenic cytokines especially HGF, IL8, angiogenin, angiopoitin-2, μPAR, CXCL1, IL-1β, IL-1α, CSF2, CSF3, MCP-3, CTACK, and OPG were found to be significantly increased in VCAM-1(+) CV-MSCs. Moreover, VCAM-1(+)CV-MSCs showed remarkable vasculo-angiogenic abilities by angiogenesis analysis with Matrigel in vitro and in vivo and the conditioned medium of VCAM-1(+) CV-MSCs exerted markedly pro-proliferative and pro-migratory effects on endothelial cells compared to VCAM-1(-)CV-MSCs. Finally, transplantation of VCAM-1(+)CV-MSCs into the ischemic hind limb of BALB/c nude mice resulted in a significantly functional improvement in comparison with VCAM-1(-)CV-MSCs transplantation.

CONCLUSIONS:

VCAM-1(+)CV-MSCs possessed a favorable angiogenic paracrine activity and displayed therapeutic efficacy on hindlimb ischemia. Our results suggested that VCAM-1(+)CV-MSCs may represent an important subpopulation of MSC for efficient therapeutic angiogenesis.

KEYWORDS:

Angiogenesis; Mesenchymal stem cells; Paracrine; Placenta; Vascular cell adhesion molecule-1 (CD106)

PMID:
27044487
PMCID:
PMC4820943
DOI:
10.1186/s13287-016-0297-0
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center