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Int J Mol Sci. 2016 Mar 29;17(4):466. doi: 10.3390/ijms17040466.

Anti-Apoptotic and Pro-Survival Effect of Alpinate Oxyphyllae Fructus (AOF) in a d-Galactose-Induced Aging Heart.

Chang YM1,2,3, Chang HH4,5,6, Kuo WW7, Lin HJ8,9, Yeh YL10,11, Padma Viswanadha V12, Tsai CC13,14, Chen RJ15, Chang HN16, Huang CY17,18,19.

Author information

1
The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung 84001, Taiwan. dnas5728@yahoo.com.tw.
2
Chinese Medicine Department, E-DA Hospital, Kaohsiung 82445, Taiwan. dnas5728@yahoo.com.tw.
3
1PT Biotechnology Co., Ltd., Taichung 433, Taiwan. dnas5728@yahoo.com.tw.
4
Research Center for Chinese Medicine & Acupuncture, China Medical University, Taichung 40402, Taiwan. glactose@hotmail.com.
5
Departments of Chinese Medicine, China Medical University Hospital, Taichung 40447, Taiwan. glactose@hotmail.com.
6
School of Post-Baccalaureate Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan. glactose@hotmail.com.
7
Department of Biological Science and Technology, China Medical University, Taichung 40447, Taiwan. wwkuo@mail.cmu.edu.tw.
8
Departments of Chinese Medicine, China Medical University Hospital, Taichung 40447, Taiwan. kawabunga0809@yahoo.com.tw.
9
School of Post-Baccalaureate Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan. kawabunga0809@yahoo.com.tw.
10
Department of pathology, Changhua Christian Hospital, Changhua 50506, Taiwan. 1867@cch.org.tw.
11
Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli 35665, Taiwan. 1867@cch.org.tw.
12
Department of Biotechnology, Bharathiar University, Coimbatore 641046, India. padma.vijaya@gmail.com.
13
The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung 84001, Taiwan. gimmy09200430@yahoo.com.tw.
14
Chinese Medicine Department, E-DA Hospital, Kaohsiung 82445, Taiwan. gimmy09200430@yahoo.com.tw.
15
Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11042, Taiwan. rayjchen@tmu.edu.tw.
16
Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan. dadalidada0809@gmail.com.
17
Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan. cyhuang@mail.cmu.edu.tw.
18
School of Chinese Medicine, China Medical University, Taichung 40447, Taiwan. cyhuang@mail.cmu.edu.tw.
19
Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan. cyhuang@mail.cmu.edu.tw.

Abstract

Aging, a natural biological/physiological phenomenon, is accelerated by reactive oxygen species (ROS) accumulation and identified by a progressive decrease in physiological function. Several studies have shown a positive relationship between aging and chronic heart failure (HF). Cardiac apoptosis was found in age-related diseases. We used a traditional Chinese medicine, Alpinate Oxyphyllae Fructus (AOF), to evaluate its effect on cardiac anti-apoptosis and pro-survival. Male eight-week-old Sprague-Dawley (SD) rats were segregated into five groups: normal control group (NC), d-Galactose-Induced aging group (Aging), and AOF of 50 (AL (AOF low)), 100 (AM (AOF medium)), 150 (AH (AOF high)) mg/kg/day. After eight weeks, hearts were measured by an Hematoxylin-Eosin (H&E) stain, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-assays and Western blotting. The experimental results show that the cardiomyocyte apoptotic pathway protein expression increased in the d-Galactose-Induced aging groups, with dose-dependent inhibition in the AOF treatment group (AL, AM, and AH). Moreover, the expression of the pro-survival p-Akt (protein kinase B (Akt)), Bcl-2 (B-cell lymphoma 2), anti-apoptotic protein (Bcl-xL) protein decreased significantly in the d-Galactose-induced aging group, with increased performance in the AOF treatment group with levels of p-IGFIR and p-PI3K (Phosphatidylinositol-3' kinase (PI3K)) to increase by dosage and compensatory performance. On the other hand, the protein of the Sirtuin 1 (SIRT1) pathway expression decreased in the aging groups and showed improvement in the AOF treatment group. Our results suggest that AOF strongly works against ROS-induced aging heart problems.

KEYWORDS:

">d-galactose-induced aging; AOF; SIRT1; apoptosis

PMID:
27043531
PMCID:
PMC4848922
DOI:
10.3390/ijms17040466
[Indexed for MEDLINE]
Free PMC Article

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