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Biosens Bioelectron. 2016 Aug 15;82:93-8. doi: 10.1016/j.bios.2016.03.036. Epub 2016 Mar 26.

A reversible and highly selective fluorescence "on-off-on" probe for detecting nickel ion in the mitochondria of living cells.

Author information

1
College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China.
2
Center of Stem Cell Research and Transformation Medicine, Anhui University, Hefei 230601, PR China.
3
College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China. Electronic address: zhpzhp@263.net.

Abstract

A simple molecular fluorescent probe L has been designed, synthesized and characterized. The probe upon interaction with different metals ions show high selectivity and sensitivity for Ni(2+) through fluorescence "turn-off" response in acetonitrile, and the fluorescence could be recovered when chelating agent TPEN is added in this system, viz an "on-off-on" process. Its recognition mechanism has been established by fluorescence spectra, (1)H NMR study. Moreover, to further explore its utility in biological system, L is selected as a probe for Ni(2+) indicator in live cells and shows sensitivity on Ni(2+) intracellular distribution. Fluorescence co-localization studies demonstrate that L is a specific mitochondria-targeted fluorescent probe with excellent cell membrane permeability. Furthermore, a rapidly reversible fluorescence imaging of probe L detecting Ni(2+) in the mitochondria is successfully achieved. These facts would make this probe a great potential for detecting Ni(2+) in biology.

KEYWORDS:

Biological application; Fluorescence probe; Mitochondria; Nickel ion; Recognition mechanism

PMID:
27043479
DOI:
10.1016/j.bios.2016.03.036
[Indexed for MEDLINE]

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