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Nutr Cancer. 2016 May-Jun;68(4):611-21. doi: 10.1080/01635581.2016.1154577. Epub 2016 Apr 4.

Young Barley Indicates Antitumor Effects in Experimental Breast Cancer In Vivo and In Vitro.

Author information

1
a Department of Medical Biology , Jessenius Faculty of Medicine, Comenius University in Bratislava , Martin , Slovakia.
2
b Department of Pharmacology , Faculty of Medicine, P. J. Šafárik University , Košice , Slovakia.
3
c Department of Pathology , Slovak Medical University and St. Elisabeth Oncology Institute , Bratislava.
4
d Laboratory of Structural Biology and Proteomics, Central Laboratories, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences , Brno , Czech Republic.
5
e Department of Anatomy , Jessenius Faculty of Medicine, Comenius University in Bratislava , Martin , Slovakia.
6
f Department of Natural Drugs, Department of Molecular Biology and Pharmaceutical Biotechnology , Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno , Czech Republic.
7
g Department of Quality of Agricultural Products , Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague , Prague , Czech Republic.
8
h Institute of Experimental Botany AS CR, v. v. i. , Prague , Czech Republic.
9
i The Centre for Chronic Disease Prevention & Management (CCDPM), College of Health & Biomedicine, Victoria University , Melbourne , Victoria , Australia.
10
j Department of Medical Biochemistry , Jessenius Faculty of Medicine, Comenius University in Bratislava , Martin , Slovakia.
11
k Department of Histology and Embryology , Jessenius Faculty of Medicine, Comenius University in Bratislava , Martin , Slovakia.
12
l Department of Veterans Affairs , Washington DC , USA.

Abstract

The effect of dietary administered young barley containing a mixture of phytochemicals to female rats for the prevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis was evaluated. After carcinogen administration (14 wk), mammary tumors were removed and prepared for histopathological and immunohistochemical analysis. Moreover, in vitro evaluation of possible mechanisms in MCF-7 breast cancer cell line was performed. Barley (0.3%) demonstrated mild antitumor effect in mammary carcinogenesis, yet 3% barley did not further improve this effect. Immunohistochemical analysis of rat tumor cells in treated groups showed significant increase in caspase-3 expression and significant reduction in Ki67 expression. In addition, 3% barley significantly decreased dityrosine levels versus control. Barley in higher dose significantly decreased serum low-density lipoprotein-cholesterol in rats. In vitro studies showed that barley significantly decreased survival of MCF-7 cells in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and significantly decreased 5-bromo-20-deoxyuridine incorporation versus control. Barley prevented cell cycle progression and extended incubation with barley showed significant increase in the percentage of annexin V/propidium iodide-positive MCF-7 cells. Our results propose an antitumor effect for the mixture of phytochemicals present in young barley in a breast cancer model.

PMID:
27042893
DOI:
10.1080/01635581.2016.1154577
[Indexed for MEDLINE]

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