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Sci Rep. 2016 Apr 4;6:23885. doi: 10.1038/srep23885.

Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche.

Author information

1
Divisions of Regenerative Medicine and Hematology-Oncology, Department of Medicine, Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093-0820, USA.
2
Biological Sciences Department, California Polytechnic State University, San Luis Obispo, CA, 93407, USA.
3
Doctoral School of Molecular and Translational Medicine, Department of Health Sciences, University of Milan, Milan, Italy.
4
Canada's Michael Smith Genome Sciences Centre, BC Cancer Agency, Vancouver, BC, Canada.
5
Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, Wako-city, Saitama, Japan.

Abstract

While implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined niche coupled with cell cycle gene expression analysis. We have identified key differences in cell cycle regulatory gene expression and transit times between normal and chronic myeloid leukemia progenitors that may inform cancer stem cell eradication strategies.

PMID:
27041210
PMCID:
PMC4819192
DOI:
10.1038/srep23885
[Indexed for MEDLINE]
Free PMC Article

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