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Neurochem Res. 2016 Aug;41(8):1868-76. doi: 10.1007/s11064-016-1898-9. Epub 2016 Apr 2.

(-)-Epigallocatechin Gallate Inhibits Asymmetric Dimethylarginine-Induced Injury in Human Brain Microvascular Endothelial Cells.

Author information

1
Department of Neurosurgery, Tianjin Nankai Hospital, Tianjin, 300100, China.
2
Department of Neurosurgery, Tianjin Nankai Hospital, Tianjin, 300100, China. changlinzhoutj@sina.com.
3
Surgical Department, Tianjin Nankai Hospital, Tianjin, 300100, China. changlinzhoutj@sina.com.

Abstract

(-)-Epigallocatechin gallate (EGCG) is the main polyphenol component of green tea (leaves of the Camellia sinensis plant). EGCG has been reported to protect human brain microvascular endothelial cells (HBMECs) against injury in several models. However, the exact mechanism is still unclear. In the current study we found that EGCG protected against asymmetric dimethylarginine (ADMA)-induced HBMEC injury, and inhibited ADMA-induced reactive oxygen species production and malondialdehyde expression. At the same time, we found that pretreatment with EGCG attenuated the upregulation of Bax and the downregulation of Bcl-2, thus confirming the cellular protective properties of EGCG against ADMA-induced apoptosis. Furthermore, we found that EGCG inhibited ADMA-induced phosphorylation of ERK1/2 and p-38, whose inhibitors relieved HBMEC injury. In conclusion, EGCG can protect against ADMA-induced HBMEC injury via the ERK1/2 and p38 MAPK pathways, which are involved in the underlying mechanisms of HBMEC injury in cerebral infarction.

KEYWORDS:

(−)-Epigallocatechin gallate; Asymmetric dimethylarginine; Cerebral infarction; Human brain microvascular endothelial cells; Mitogen-activated protein kinases

PMID:
27038929
DOI:
10.1007/s11064-016-1898-9
[Indexed for MEDLINE]

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