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Ultrasound Med Biol. 2016 Jun;42(6):1375-84. doi: 10.1016/j.ultrasmedbio.2016.01.018. Epub 2016 Mar 31.

Species-Independent Modeling of High-Frequency Ultrasound Backscatter in Hyaline Cartilage.

Author information

1
Berlin-Brandenburg Center for Regenerative Therapies and Berlin-Brandenburg School for Regenerative Therapies, Charité-Universitätsmedizin Berlin, Berlin, Germany.
2
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
3
Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Berlin, Germany.
4
Bioacoustics Research Laboratory, Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
5
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland; Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland.
6
Berlin-Brandenburg Center for Regenerative Therapies and Berlin-Brandenburg School for Regenerative Therapies, Charité-Universitätsmedizin Berlin, Berlin, Germany. Electronic address: kay.raum@charite.de.

Abstract

Apparent integrated backscatter (AIB) is a common ultrasound parameter used to assess cartilage matrix degeneration. However, the specific contributions of chondrocytes, proteoglycan and collagen to AIB remain unknown. To reveal these relationships, this work examined biopsies and cross sections of human, ovine and bovine cartilage with 40-MHz ultrasound biomicroscopy. Site-matched estimates of collagen concentration, proteoglycan concentration, collagen orientation and cell number density were employed in quasi-least-squares linear regression analyses to model AIB. A positive correlation (R(2) = 0.51, p < 10(-4)) between AIB and a combination model of cell number density and collagen concentration was obtained for collagen orientations approximately perpendicular (>70°) to the sound beam direction. These findings indicate causal relationships between AIB and cartilage structural parameters and could aid in more sophisticated future interpretations of ultrasound backscatter.

KEYWORDS:

Apparent integrated backscatter; Backscatter; Backscatter coefficient; Cartilage; Osteoarthritis; Quantitative ultrasound; Ultrasound; Ultrasound spectroscopy

[Indexed for MEDLINE]

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