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Chembiochem. 2016 May 17;17(10):900-3. doi: 10.1002/cbic.201600068. Epub 2016 Apr 1.

Multiplexed Imaging of Protein Phosphorylation on Membranes Based on Ti(IV) Functionalized Nanopolymers.

Author information

1
Department of Biochemistry, Purdue University, 201 S University, West Lafayette, IN, 47907, USA.
2
Tymora Analytical Operations, 3495 Kent Avenue, West Lafayette, IN, 47906, USA.
3
Department of Biochemistry, Purdue University, 201 S University, West Lafayette, IN, 47907, USA. watao@purdue.edu.
4
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN, 47907, USA. watao@purdue.edu.

Abstract

Accurate protein phosphorylation analysis reveals dynamic cellular signaling events not evident from protein expression levels. The most dominant biochemical assay, western blotting, suffers from the inadequate availability and poor quality of phospho-specific antibodies for phosphorylated proteins. Furthermore, multiplexed assays based on antibodies are limited by steric interference between the antibodies. Here we introduce a multifunctionalized nanopolymer for the universal detection of phosphoproteins that, in combination with regular antibodies, allows multiplexed imaging and accurate determination of protein phosphorylation on membranes.

KEYWORDS:

antibodies; dendrimers; membranes; multiplexed analysis; phosphoproteins

PMID:
27037847
PMCID:
PMC4870103
DOI:
10.1002/cbic.201600068
[Indexed for MEDLINE]
Free PMC Article

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