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Cold Spring Harb Protoc. 2016 Apr 1;2016(4):pdb.prot084814. doi: 10.1101/pdb.prot084814.

Isolating Lysosomes from Rat Liver.

Author information

1
Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York, York YO10 5DD, United Kingdom.

Abstract

This protocol describes the generation of a fraction enriched in lysosomes from rat liver. The lysosomes are rapidly isolated using density-gradient centrifugation with gradient media that retain the osmolarity of the lysosomes such that they are functional and can be used in in vitro assays.

PMID:
27037067
DOI:
10.1101/pdb.prot084814
[Indexed for MEDLINE]

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