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Biochim Biophys Acta. 2016 Jul;1860(7):1388-94. doi: 10.1016/j.bbagen.2016.02.019. Epub 2016 Mar 28.

New partners and phosphorylation sites of focal adhesion kinase identified by mass spectrometry.

Author information

1
Developmental Neurobiology and Neural Regeneration Group, Department of Cell Biology, Faculty of Biology, University of Barcelona, Diagonal 643, 08038 Barcelona, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, 28031 Madrid, Spain.
2
Developmental Neurobiology and Neural Regeneration Group, Department of Cell Biology, Faculty of Biology, University of Barcelona, Diagonal 643, 08038 Barcelona, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, 28031 Madrid, Spain; Vall d´Hebron Institute of Research, 08035 Barcelona, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA), 08010 Barcelona, Spain.
3
Developmental Neurobiology and Neural Regeneration Group, Department of Cell Biology, Faculty of Biology, University of Barcelona, Diagonal 643, 08038 Barcelona, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, 28031 Madrid, Spain. Electronic address: fburgaya@ub.edu.

Abstract

The regulation of focal adhesion kinase (FAK) involves phosphorylation and multiple interactions with other signaling proteins. Some of these pathways are relevant for nervous system functions such as branching, axonal guidance, and plasticity. In this study, we screened mouse brain to identify FAK-interactive proteins and phosphorylatable residues as a first step to address the neuronal functions of this kinase. Using mass spectrometry analysis, we identified new phosphorylated sites (Thr 952, Thr 1048, and Ser 1049), which lie in the FAT domain; and putative new partners for FAK, which include cytoskeletal proteins such as drebrin and MAP 6, adhesion regulators such as neurabin-2 and plakophilin 1, and synapse-associated proteins such as SynGAP and a NMDA receptor subunit. Our findings support the participation of brain-localized FAK in neuronal plasticity.

KEYWORDS:

Brain; Focal adhesion kinase; Mass spectrometry; Synapse

PMID:
27033120
DOI:
10.1016/j.bbagen.2016.02.019
[Indexed for MEDLINE]

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