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Nat Commun. 2016 Mar 31;7:11095. doi: 10.1038/ncomms11095.

Identification of plant vacuolar transporters mediating phosphate storage.

Author information

1
Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Section 2, Taipei 11529, Taiwan.
2
Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica, Taipei 11529, Taiwan.
3
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 40243, Taiwan.
4
Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan.
5
Graduate Institute of Applied Physics, National Chengchi University, Taipei 11605, Taiwan.
6
Biotechnology Center, National Chung-Hsing University, Taichung 40243, Taiwan.

Abstract

Plant vacuoles serve as the primary intracellular compartments for inorganic phosphate (Pi) storage. Passage of Pi across vacuolar membranes plays a critical role in buffering the cytoplasmic Pi level against fluctuations of external Pi and metabolic activities. Here we demonstrate that the SPX-MFS proteins, designated as PHOSPHATE TRANSPORTER 5 family (PHT5), also named Vacuolar Phosphate Transporter (VPT), function as vacuolar Pi transporters. Based on (31)P-magnetic resonance spectroscopy analysis, Arabidopsis pht5;1 loss-of-function mutants accumulate less Pi and exhibit a lower vacuolar-to-cytoplasmic Pi ratio than controls. Conversely, overexpression of PHT5 leads to massive Pi sequestration into vacuoles and altered regulation of Pi starvation-responsive genes. Furthermore, we show that heterologous expression of the rice homologue OsSPX-MFS1 mediates Pi influx to yeast vacuoles. Our findings show that a group of Pi transporters in vacuolar membranes regulate cytoplasmic Pi homeostasis and are required for fitness and plant growth.

PMID:
27029856
PMCID:
PMC4821872
DOI:
10.1038/ncomms11095
[Indexed for MEDLINE]
Free PMC Article

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