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Adv Healthc Mater. 2016 Jul;5(13):1554-9. doi: 10.1002/adhm.201500981. Epub 2016 Mar 29.

Efficient Purification and Release of Circulating Tumor Cells by Synergistic Effect of Biomarker and SiO2 @Gel-Microbead-Based Size Difference Amplification.

Author information

1
Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education School of Physics and Technology, Wuhan University, Wuhan, 430072, Hubei, P. R. China.
2
Institute for Interdisciplinary Research, Jianghan University, Wuhan, 430056, China.
3
Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA, 16802, USA.
4
Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry Chinese Academy of Science, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China.

Abstract

Microfluidics-based circulating tumor cell (CTC) isolation is achieved by using gelatin-coated silica microbeads conjugated to CTC-specific antibodies. Bead-binding selectively enlarges target cell size, providing efficient high-purity capture. CTCs captured can be further released non-invasively. This stratagem enables high-performance CTC isolation for subsequent studies.

KEYWORDS:

cell capture; cell release; circulating tumor cells; microfluidics; size amplification

PMID:
27028055
DOI:
10.1002/adhm.201500981
[Indexed for MEDLINE]

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