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Mol Nutr Food Res. 2016 Sep;60(9):1912-23. doi: 10.1002/mnfr.201501008. Epub 2016 May 23.

Comparison of anti-inflammatory mechanisms of mango (Mangifera Indica L.) and pomegranate (Punica Granatum L.) in a preclinical model of colitis.

Author information

1
Department of Nutrition and Food Science, Texas A&M University, College Station, TX, USA.
2
Interdisciplinary Program of Toxicology, Texas A&M University, College Station, TX, USA.
3
Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, USA.
4
Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, USA.
5
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, USA.
6
Center for Epigenetics & Disease Prevention, Texas A&M Health Science Center, Houston, TX, USA.
7
Department of Nutrition and Food Science, Texas A&M University, College Station, TX, USA. smtalcott@tamu.edu.
8
Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, USA. smtalcott@tamu.edu.

Abstract

SCOPE:

Tannin-rich fruits have been evaluated as alternative prevention strategies for colorectal cancer based on their anti-inflammatory properties. This study compared tannin-rich preparations from mango (rich in gallotannins) and pomegranate (rich in ellagitannins) in the dextran sodium sulfate-induced colitis model.

METHODS AND RESULTS:

In rats, mango and pomegranate beverages decreased intestinal inflammation and the levels of pro-inflammatory cytokines in mucosa and serum. The mango beverage suppressed the ratio of phosphorylated/total protein expression of the IGF-1R-AKT/mTOR axis and downregulated mRNA expression of Igf1, Insr, and pik3cv. Pomegranate decreased p70S6K and RPS6, as well as Rps6ka2, Map2k2, and Mapk1 mRNA. In silico modeling indicated a high binding of docked of gallic acid to the catalytic domain of IGF-1R, which may suppress the activity of the enzyme. Ellagic acid docked effectively into the catalytic domains of both IGF-1R and EGFR. In vitro assays with lipopolysaccharide-treated CCD-18Co cells using polyphenolic extracts from each beverage, as well as pure compounds, corroborated the predictions made in silico.

CONCLUSION:

Mango polyphenols inhibited the IGF-1R- AKT/mTOR axis, and pomegranate polyphenols downregulate the mTOR downstream pathway through reductions in ERK1/2. These results suggest that extracts rich in gallo- and ellagitannins act on different molecular targets in the protection against ulcerative colitis.

KEYWORDS:

Colitis Mango mTOR pathway Pomegranate Rat

PMID:
27028006
PMCID:
PMC5026564
DOI:
10.1002/mnfr.201501008
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

The authors have declared no conflict of interest.

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