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Mol Neurobiol. 2017 May;54(4):2901-2921. doi: 10.1007/s12035-016-9842-1. Epub 2016 Mar 29.

An Antagomir to MicroRNA-106b-5p Ameliorates Cerebral Ischemia and Reperfusion Injury in Rats Via Inhibiting Apoptosis and Oxidative Stress.

Author information

1
Department of Clinical Laboratory, Jiangsu Province Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, Jiangsu, China.
2
The Second Clinical College, Nanjing University of Chinese Medicine, Nanjing, 210046, Jiangsu, China.
3
Basic Medical Sciences, Nanjing University of Chinese Medicine, Nanjing, 210046, Jiangsu, China.
4
Department of Clinical Laboratory, Jiangsu Province Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, Jiangsu, China. zzzzcb99@163.com.
5
Basic Medical Sciences, Nanjing University of Chinese Medicine, Nanjing, 210046, Jiangsu, China. zzzzcb99@163.com.

Abstract

We previously observed that microRNA miR-106b-5p significantly increased in serum of patients with acute ischemic stroke. The present study was to determine whether miR-106b-5p antagomir can protect against cerebral ischemia/reperfusion (I/R) injury and elucidate its underlying mechanisms. Middle cerebral artery occlusion (MCAO) was operated on male Sprague Dawley rats. MiR-106b-5p antagomir significantly decreased neurological deficit scores, infarct volumes, and neuronal injury. Furthermore, miR-106b-5p antagomir markedly reduced malondialdehyde (MDA) content, restored superoxide dismutase (SOD) activity, increased the expression of myeloid cell leukemia-1 (Mcl-1) and B cell lymphoma-2 (Bcl-2), and decreased the expression of Bax in the ischemic cortex. In PC12 cells, miR-106b-5p inhibitor increased the Mcl-1 and Bcl-2 expression, which provided protection against glutamate-induced apoptosis and oxidative damage, as evidenced by decreased lactate dehydrogenase (LDH) release, and enhanced SOD activity. Notably, luciferase reported assay proved Mcl-1 was the target gene of miR-106b-5p. In conclusion, our data indicates that the neuroprotective effects of miR-106b-5p antagomir on cerebral I/R injury are associated with its inhibition of apoptosis and oxidative stress, suggesting a potential therapeutic target for ischemic stroke.

KEYWORDS:

Apoptosis; Ischemic stroke; MiR-106b-5p; Middle cerebral artery occlusion; Oxidative stress

PMID:
27023223
DOI:
10.1007/s12035-016-9842-1
[Indexed for MEDLINE]

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