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Allergol Int. 2016 Jul;65(3):228-34. doi: 10.1016/j.alit.2016.02.008. Epub 2016 Mar 26.

Novel insights into cutaneous immune systems revealed by in vivo imaging.

Author information

1
Department of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, Japan. Electronic address: hontetsu@kuhp.kyoto-u.ac.jp.
2
Department of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
3
Department of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, Japan; Singapore Immunology Network (SIgN) and Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore; PRESTO, Japan Science and Technology Agency, Saitama, Japan. Electronic address: kaba@kuhp.kyoto-u.ac.jp.

Abstract

In vivo imaging is a novel experimental approach for biological research. Multiphoton microscopy (MPM), a type of fluorescence microscopy, is a new tool for in vivo imaging analysis. MPM allows observation of both tissue structures and cell behaviors or cell-cell interactions in living animals in real time. Skin is an ideal tissue for MPM analysis as it is directly accessible to the microscope. In the skin, immune cells cooperate to maintain skin homeostasis or to exert immune responses against foreign antigens. In vivo imaging by MPM analysis provides precise information on cell dynamics in the skin, and has significantly expanded our knowledge of the cutaneous immune system. In this review, we will discuss recent insights related to the mechanisms of allergic skin inflammation that have been revealed by MPM analysis.

KEYWORDS:

Contact hypersensitivity; Dendritic cells; In vivo imaging; Multiphoton microscopy; T cells

PMID:
27021658
DOI:
10.1016/j.alit.2016.02.008
[Indexed for MEDLINE]
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