Format

Send to

Choose Destination
J Lipid Res. 2016 Jun;57(6):1017-28. doi: 10.1194/jlr.M066175. Epub 2016 Mar 28.

Alcohol produces distinct hepatic lipidome and eicosanoid signature in lean and obese.

Author information

1
Division of Gastroenterology, Hepatology and Nutrition, Department of Internal Medicine, Virginia Commonwealth University School of Medicine, Richmond, VA puneet.puri@vcuhealth.org.
2
University of Southern California, Los Angeles, CA.
3
Zora Biosciences Oy, Espoo, Finland.
4
Division of Gastroenterology, Hepatology and Nutrition, Department of Internal Medicine, Virginia Commonwealth University School of Medicine, Richmond, VA.
5
Venebio Group LLC, Richmond, VA.

Abstract

Alcohol- and obesity-related liver diseases often coexist. The hepatic lipidomics due to alcohol and obesity interaction is unknown. We characterized the hepatic lipidome due to 1) alcohol consumption in lean and obese mice and 2) obesity and alcohol interactions. In the French-Tsukamoto mouse model, intragastric alcohol or isocaloric dextrose were fed with either chow (lean) or high-fat, high-cholesterol diet (obese). Four groups (lean, lean alcohol, obese, and obese alcohol) were studied. MS was performed for hepatic lipidomics, and data were analyzed. Alcohol significantly increased hepatic cholesteryl esters and diacyl-glycerol in lean and obese but was more pronounced in obese. Alcohol produced contrasting changes in hepatic phospholipids with significant enrichment in lean mice versus significant decrease in obese mice, except phosphatidylglycerol, which was increased in both lean and obese alcohol groups. Most lysophospholipids were increased in lean alcohol and obese mice without alcohol use only. Prostaglandin E2; 5-, 8-, and 11-hydroxyeicosatetraenoic acids; and 9- and 13-hydroxyoctadecadienoic acids were considerably increased in obese mice with alcohol use. Alcohol consumption produced distinct changes in lean and obese with profound effects of obesity and alcohol interaction on proinflammatory and oxidative stress-related eicosanoids.

KEYWORDS:

cyclooxygenase; cytochrome P450; hydroxyeicosatetraenoic acids; lipoxygenase; liver; obesity; phospholipids; prostaglandins

PMID:
27020313
PMCID:
PMC4878186
DOI:
10.1194/jlr.M066175
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center