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Mol Plant Pathol. 2017 Apr;18(3):378-390. doi: 10.1111/mpp.12406. Epub 2016 May 26.

Quantitative proteomics links metabolic pathways to specific developmental stages of the plant-pathogenic oomycete Phytophthora capsici.

Author information

1
Department of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, China.
2
Division of Glycoscience, Royal Institute of Technology (KTH), AlbaNova University Centre, Stockholm, SE-10691, Sweden.
3
Australian Research Council Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.

Abstract

The oomycete Phytophthora capsici is a plant pathogen responsible for important losses to vegetable production worldwide. Its asexual reproduction plays an important role in the rapid propagation and spread of the disease in the field. A global proteomics study was conducted to compare two key asexual life stages of P. capsici, i.e. the mycelium and cysts, to identify stage-specific biochemical processes. A total of 1200 proteins was identified using qualitative and quantitative proteomics. The transcript abundance of some of the enriched proteins was also analysed by quantitative real-time polymerase chain reaction. Seventy-three proteins exhibited different levels of abundance between the mycelium and cysts. The proteins enriched in the mycelium are mainly associated with glycolysis, the tricarboxylic acid (or citric acid) cycle and the pentose phosphate pathway, providing the energy required for the biosynthesis of cellular building blocks and hyphal growth. In contrast, the proteins that are predominant in cysts are essentially involved in fatty acid degradation, suggesting that the early infection stage of the pathogen relies primarily on fatty acid degradation for energy production. The data provide a better understanding of P. capsici biology and suggest potential metabolic targets at the two different developmental stages for disease control.

KEYWORDS:

Phytophthora capsici; cysts; mass spectrometry; mycelium; plant-pathogenic oomycete; quantitative proteomics

PMID:
27019332
DOI:
10.1111/mpp.12406
[Indexed for MEDLINE]

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