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Tissue Eng Part A. 2016 Apr;22(7-8):698-706. doi: 10.1089/ten.TEA.2015.0461.

Nanografted Substrata and Triculture of Human Pericytes, Fibroblasts, and Endothelial Cells for Studying the Effects on Angiogenesis.

Kim TH1,2, Kim SH1,2,3, Leong KW4, Jung Y1,3.

Author information

1
1 Biomaterials Research Center, Korea Institute of Science and Technology , Seoul, Republic of Korea .
2
2 NBIT, KU-KIST Graduate School of Converging Science and Technology, Korea University , Seoul, Republic of Korea .
3
3 Department of Biomedical Engineering, Korea University of Science and Technology (UST), Daejeon, Republic of Korea .
4
4 Department of Biomedical Engineering, Columbia University , New York, New York.

Abstract

For the successful treatment of severe wounds, angiogenesis must be induced to provide a sufficient blood supply to the wound site. There have been many studies on various structural and biochemical factors regulating angiogenesis, such as surface topography, surface modifications, angiogenic factors, and various cell types. However, there is a paucity of research on the effects of micro- and nanoscale topography and the application of pericytes in angiogenesis. In this study, we utilized nanoscale topography combined with a triculture system consisting of human pericytes, fibroblasts, and endothelial cells. We investigated the effects of the nanografted substrata and a triculture system on proangiogenic therapies in vitro. Human dermal fibroblasts and human umbilical vein endothelial cells were seeded with human pericytes from the placenta directly onto nanografted substrata composed of polydimethylsiloxane. We demonstrated that key elements of angiogenesis, including segment and capillary-like tubular structure formation, as well as the secretion of extracellular matrix, were increased by interaction between the nanografted substrata and the coculture containing pericytes. Thus, nanografted surfaces and triculture systems containing human pericytes, fibroblasts, and endothelial cells promote angiogenesis.

PMID:
27019156
DOI:
10.1089/ten.TEA.2015.0461
[Indexed for MEDLINE]

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