Format

Send to

Choose Destination
Anal Chem. 2016 Jun 21;88(12):6254-64. doi: 10.1021/acs.analchem.6b00195. Epub 2016 Apr 5.

Polydimethylsiloxane-Paper Hybrid Lateral Flow Assay for Highly Sensitive Point-of-Care Nucleic Acid Testing.

Choi JR1,2,3, Liu Z3,4, Hu J1,3, Tang R1,3,5,6, Gong Y1,3, Feng S3,7,8, Ren H3,9, Wen T10, Yang H5,6, Qu Z4, Pingguan-Murphy B2, Xu F1,3.

Author information

1
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University , Xi'an, Shaanxi 710049, PR China.
2
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya , Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
3
Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University , Xi'an, Shaanxi 710049, PR China.
4
Key Laboratory of Thermo-Fluid Science and Engineering of Ministry of Education, School of Energy and Power Engineering, Xi'an Jiaotong University , Xi'an, Shaanxi 710049, PR China.
5
School of Life Sciences, Northwestern Polytechnical University , Xi'an, Shaanxi 710072, PR China.
6
Key Laboratory of Space Bioscience and Biotechnology, Northwestern Polytechnical University , Xi'an, Shaanxi 710072, PR China.
7
MOE Key Laboratory of Multifunctional Materials and Structures (LMMS), School of Aerospace, Xi'an Jiaotong University , Xi'an, Shaanxi 710049, PR China.
8
State Key Laboratory of Mechanical Structure Strength and Vibration, School of Aerospace, Xi'an Jiaotong University , Xi'an, Shaanxi 710049, PR China.
9
Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an, Shaanxi 710061, PR China.
10
Xi'an Diandi Biotech Company , Xi'an, Shaanxi 710049, PR China.

Abstract

In nucleic acid testing (NAT), gold nanoparticle (AuNP)-based lateral flow assays (LFAs) have received significant attention due to their cost-effectiveness, rapidity, and the ability to produce a simple colorimetric readout. However, the poor sensitivity of AuNP-based LFAs limits its widespread applications. Even though various efforts have been made to improve the assay sensitivity, most methods are inappropriate for integration into LFA for sample-to-answer NAT at the point-of-care (POC), usually due to the complicated fabrication processes or incompatible chemicals used. To address this, we propose a novel strategy of integrating a simple fluidic control strategy into LFA. The strategy involves incorporating a piece of paper-based shunt and a polydimethylsiloxane (PDMS) barrier to the strip to achieve optimum fluidic delays for LFA signal enhancement, resulting in 10-fold signal enhancement over unmodified LFA. The phenomena of fluidic delay were also evaluated by mathematical simulation, through which we found the movement of fluid throughout the shunt and the tortuosity effects in the presence of PDMS barrier, which significantly affect the detection sensitivity. To demonstrate the potential of integrating this strategy into a LFA with sample-in-answer-out capability, we further applied this strategy into our prototype sample-to-answer LFA to sensitively detect the Hepatitis B virus (HBV) in clinical blood samples. The proposed strategy offers great potential for highly sensitive detection of various targets for wide application in the near future.

PMID:
27012657
DOI:
10.1021/acs.analchem.6b00195
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center