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Transgenic Res. 2016 Oct;25(5):561-73. doi: 10.1007/s11248-016-9953-5. Epub 2016 Mar 24.

CRISPR/Cas9: an advanced tool for editing plant genomes.

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Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, Kharagpur, 721302, India.
Department of Human Genetics, University of Michigan, Ann Arbor, MI, 48109, USA.


To meet current challenges in agriculture, genome editing using sequence-specific nucleases (SSNs) is a powerful tool for basic and applied plant biology research. Here, we describe the principle and application of available genome editing tools, including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeat associated CRISPR/Cas9 system. Among these SSNs, CRISPR/Cas9 is the most recently characterized and rapidly developing genome editing technology, and has been successfully utilized in a wide variety of organisms. This review specifically illustrates the power of CRISPR/Cas9 as a tool for plant genome engineering, and describes the strengths and weaknesses of the CRISPR/Cas9 technology compared to two well-established genome editing tools, ZFNs and TALENs.


CRISPR/Cas9; Genome editing; Plants; Sequence-specific nucleases; TALENs; ZFNs

[Indexed for MEDLINE]

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