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Transgenic Res. 2016 Oct;25(5):561-73. doi: 10.1007/s11248-016-9953-5. Epub 2016 Mar 24.

CRISPR/Cas9: an advanced tool for editing plant genomes.

Author information

1
Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, Kharagpur, 721302, India.
2
Department of Human Genetics, University of Michigan, Ann Arbor, MI, 48109, USA. sgayen@umich.edu.

Abstract

To meet current challenges in agriculture, genome editing using sequence-specific nucleases (SSNs) is a powerful tool for basic and applied plant biology research. Here, we describe the principle and application of available genome editing tools, including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeat associated CRISPR/Cas9 system. Among these SSNs, CRISPR/Cas9 is the most recently characterized and rapidly developing genome editing technology, and has been successfully utilized in a wide variety of organisms. This review specifically illustrates the power of CRISPR/Cas9 as a tool for plant genome engineering, and describes the strengths and weaknesses of the CRISPR/Cas9 technology compared to two well-established genome editing tools, ZFNs and TALENs.

KEYWORDS:

CRISPR/Cas9; Genome editing; Plants; Sequence-specific nucleases; TALENs; ZFNs

PMID:
27012546
DOI:
10.1007/s11248-016-9953-5
[Indexed for MEDLINE]

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