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J Environ Radioact. 2016 Jun;157:60-6. doi: 10.1016/j.jenvrad.2016.03.003. Epub 2016 Mar 21.

Subcellular distribution and chemical forms of thorium in Brassica juncea var. foliosa.

Author information

1
College of Chemistry, Sichuan University, Chengdu 610064, China.
2
Logistic Engineering University, Chongqing 401311, China.
3
Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang 621900, China.
4
Logistic Engineering University, Chongqing 401311, China. Electronic address: fxjhg@126.com.
5
College of Chemistry, Sichuan University, Chengdu 610064, China. Electronic address: jinyongdong@scu.edu.cn.
6
College of Chemistry, Sichuan University, Chengdu 610064, China. Electronic address: xiachqin@163.com.

Abstract

Brassica juncea var. foliosa (B. juncea var. foliosa) is a promising species for thorium (Th) phytoextraction due to its large biomass, fast growth rate and high tolerance toward Th. To further understand the mechanisms of Th tolerance, the present study investigated the subcellular distribution and chemical forms of Th found in B. juncea var. foliosa Our results indicated that in both roots and leaves, Th contents in different parts of the cells follow the order of cell wall > membranes and soluble fraction > organelles. In particular, Transmission Electron Microscope (TEM) analysis showed that Th was abundantly located in cell walls of the roots. Additionally, when plants were exposed to different concentrations of Th, we have found that Th existed in B. juncea var. foliosa with different chemical forms. Much of the Th extracted by 2% acetic acid (HAc), 1 M NaCl and HCl in roots with the percentage distribution varied from 47.2% to 62.5%, while in leaves, most of the Th was in the form of residue and the subdominant amount of Th was extracted by HCl, followed by 2% HAc. This suggested that Th compartmentation in cytosol and integration with phosphate or proteins in cell wall might be responsible for the tolerance of B. juncea var. foliosa to the stress of Th.

KEYWORDS:

Chemical form; Subcellular distribution; Th; Toxicity

PMID:
27010411
DOI:
10.1016/j.jenvrad.2016.03.003
[Indexed for MEDLINE]

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