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Physiol Rep. 2016 Mar;4(6). pii: e12673. doi: 10.14814/phy2.12673.

Comparison between direct and reverse electroporation of cells in situ: a simulation study.

Author information

1
Department of Bioengineering, Imperial College London, London, United Kingdom.
2
Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland Department of Medical Specializations - Cardiology, University of Geneva, Geneva, Switzerland.
3
Department of Oncology, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland.
4
Department of Bioengineering, Imperial College London, London, United Kingdom r.krams@imperial.ac.uk.

Abstract

The discovery of the human genome has unveiled new fields of genomics, transcriptomics, and proteomics, which has produced paradigm shifts on how to study disease mechanisms, wherein a current central focus is the understanding of how gene signatures and gene networks interact within cells. These gene function studies require manipulating genes either through activation or inhibition, which can be achieved by temporarily permeabilizing the cell membrane through transfection to delivercDNAorRNAi. An efficient transfection technique is electroporation, which applies an optimized electric pulse to permeabilize the cells of interest. When the molecules are applied on top of seeded cells, it is called "direct" transfection and when the nucleic acids are printed on the substrate and the cells are seeded on top of them, it is termed "reverse" transfection. Direct transfection has been successfully applied in previous studies, whereas reverse transfection has recently gained more attention in the context of high-throughput experiments. Despite the emerging importance, studies comparing the efficiency of the two methods are lacking. In this study, a model for electroporation of cells in situ is developed to address this deficiency. The results indicate that reverse transfection is less efficient than direct transfection. However, the model also predicts that by increasing the concentration of deliverable molecules by a factor of 2 or increasing the applied voltage by 20%, reverse transfection can be approximately as efficient as direct transfection.

KEYWORDS:

Electroporation; high‐throughput techniques; transfection efficiency

PMID:
27009275
PMCID:
PMC4814886
DOI:
10.14814/phy2.12673
[Indexed for MEDLINE]
Free PMC Article

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