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Food Chem. 2016 Aug 15;205:204-11. doi: 10.1016/j.foodchem.2016.03.019. Epub 2016 Mar 8.

Phytochemicals from Camellia nitidissima Chi inhibited the formation of advanced glycation end-products by scavenging methylglyoxal.

Author information

1
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA.
2
Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA.
3
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
4
Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing 210009, China.
5
Guangxi Gui RenTang Co., Ltd, Fangchenggang 538021, China.
6
School of Nutrition and Food Science, Louisiana State University, Baton Rouge, LA 34202, USA.
7
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: aiqunj302@njust.edu.cn.

Abstract

The objective of this study was to investigate the inhibitory effects of Camellia nitidissima Chi (CNC) on the advanced glycation end-product (AGE) formation. CNC was extracted with ethanol and further separated into dichloromethane, ethyl acetate, n-butanol, and water soluble fractions. Ethyl acetate fraction had the highest total phenolic and quercetin content compared with other fractions. Sixteen phenolic compounds were identified using HPLC Triple TOF MS/MS. Bovine serum albumin (BSA)-glucose assay showed that dichloromethane and ethyl acetate fraction inhibited AGE formation by 88.1% and 87.5% at 2.5mg/mL. BSA-methylglyoxal assay showed that ethyl acetate fraction inhibited 54.1% AGE formation while dichloromethane fraction inhibited 28.1%. Over 96.0% of methylglyoxal was scavenged by different fractions within 12h. Both mono- and di-methylglyoxal quercetin adducts were identified after incubating quercetin with methylglyoxal using HPLC-ESI-MS(n). The results in this study suggest that CNC extracts inhibited AGEs formation in part through scavenging methylglyoxal by phenolic compounds.

KEYWORDS:

Advanced glycation end-products; Camellia nitidissima Chi; Quercetin; Scavenging methylglyoxal

PMID:
27006232
DOI:
10.1016/j.foodchem.2016.03.019
[Indexed for MEDLINE]

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