Phytochemicals from Camellia nitidissima Chi inhibited the formation of advanced glycation end-products by scavenging methylglyoxal

Food Chem. 2016 Aug 15:205:204-11. doi: 10.1016/j.foodchem.2016.03.019. Epub 2016 Mar 8.

Abstract

The objective of this study was to investigate the inhibitory effects of Camellia nitidissima Chi (CNC) on the advanced glycation end-product (AGE) formation. CNC was extracted with ethanol and further separated into dichloromethane, ethyl acetate, n-butanol, and water soluble fractions. Ethyl acetate fraction had the highest total phenolic and quercetin content compared with other fractions. Sixteen phenolic compounds were identified using HPLC Triple TOF MS/MS. Bovine serum albumin (BSA)-glucose assay showed that dichloromethane and ethyl acetate fraction inhibited AGE formation by 88.1% and 87.5% at 2.5mg/mL. BSA-methylglyoxal assay showed that ethyl acetate fraction inhibited 54.1% AGE formation while dichloromethane fraction inhibited 28.1%. Over 96.0% of methylglyoxal was scavenged by different fractions within 12h. Both mono- and di-methylglyoxal quercetin adducts were identified after incubating quercetin with methylglyoxal using HPLC-ESI-MS(n). The results in this study suggest that CNC extracts inhibited AGEs formation in part through scavenging methylglyoxal by phenolic compounds.

Keywords: Advanced glycation end-products; Camellia nitidissima Chi; Quercetin; Scavenging methylglyoxal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Camellia / chemistry*
  • Glycation End Products, Advanced / chemistry*
  • Phytochemicals / chemistry*
  • Plant Extracts / chemistry*
  • Pyruvaldehyde / chemistry*
  • Quercetin / chemistry*

Substances

  • Glycation End Products, Advanced
  • Phytochemicals
  • Plant Extracts
  • Pyruvaldehyde
  • Quercetin