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Nat Commun. 2016 Mar 23;7:11088. doi: 10.1038/ncomms11088.

Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy.

Author information

1
Department of Chemistry, Stony Brook University, Stony Brook, New York 11794, USA.
2
Department of Biochemistry &Cell Biology, Stony Brook University, Stony Brook, New York 11794, USA.

Abstract

Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes SPIM live imaging performance by using a real-time adjustable tiling light sheet and creating a flexible compromise between spatial and temporal resolution. We demonstrate the 3D live imaging ability of TLS-SPIM by imaging cellular and subcellular behaviours in live C. elegans and zebrafish embryos, and show how TLS-SPIM can facilitate cell biology research in multicellular specimens by studying left-right symmetry breaking behaviour of C. elegans embryos.

PMID:
27004937
PMCID:
PMC4814582
DOI:
10.1038/ncomms11088
[Indexed for MEDLINE]
Free PMC Article

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