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Infect Immun. 2016 May 24;84(6):1682-1692. doi: 10.1128/IAI.01330-15. Print 2016 Jun.

Staphylococcus aureus Phenol-Soluble Modulins Impair Interleukin Expression in Bovine Mammary Epithelial Cells.

Author information

1
INRA, UMR1253 STLO, Rennes, France.
2
Agrocampus Ouest, UMR1253 STLO, Rennes, France.
3
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation.
4
Laboratory of Bacteriology, National Institutes of Health, Bethesda, Maryland, USA.
5
INRA, UMR1348 PEGASE, Saint Gilles, France.
6
Clinic for Ruminants at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians University Munich, Munich, Germany.
7
INRA, UMR1282, Infectiologie et Santé Publique, Nouzilly, France.
8
Université François Rabelais de Tours, UMR1282, Infectiologie et Santé Publique, Tours, France.
9
Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom.
10
INRA, UMR1253 STLO, Rennes, France nadia.berkova@rennes.inra.fr.

Abstract

The role of the recently described interleukin-32 (IL-32) in Staphylococcus aureus-induced mastitis, an inflammation of the mammary gland, is unclear. We determined expression of IL-32, IL-6, and IL-8 in S. aureus- and Escherichia coli-infected bovine mammary gland epithelial cells. Using live bacteria, we found that in S. aureus-infected cells, induction of IL-6 and IL-8 expression was less pronounced than in E. coli-infected cells. Notably, IL-32 expression was decreased in S. aureus-infected cells, while it was increased in E. coli-infected cells. We identified the staphylococcal phenol-soluble modulin (PSM) peptides as key contributors to these effects, as IL-32, IL-6, and IL-8 expression by epithelial cells exposed to psm mutant strains was significantly increased compared to that in cells exposed to the isogenic S. aureus wild-type strain, indicating that PSMs inhibit the production of these interleukins. The use of genetically complemented strains confirmed this observation. Inasmuch as the decreased expression of IL-32, which is involved in dendritic cell maturation, impairs immune responses, our results support a PSM-dependent mechanism that allows for the development of chronic S. aureus-related mastitis.

PMID:
27001539
PMCID:
PMC4907149
DOI:
10.1128/IAI.01330-15
[Indexed for MEDLINE]
Free PMC Article

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