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Sci Rep. 2016 Mar 22;6:23129. doi: 10.1038/srep23129.

Human gut colonisation may be initiated in utero by distinct microbial communities in the placenta and amniotic fluid.

Author information

1
Functional Foods Forum, University of Turku, Itäinen Pitkäkatu 4A, 20520, Turku, Finland.
2
Department of Biotechnology, Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), Av Agustin Escardino 7, 49860, Paterna, Valencia, Spain.
3
Department of Paediatrics, University of Turku and Turku University Hospital, Kiinamyllynkatu 4-8, 20520, Turku, Finland.
4
Food Chemistry and Food Development, Department of Biochemistry, University of Turku, Vatselankatu 2, 20500, Turku, Finland.

Abstract

Interaction with intestinal microbes in infancy has a profound impact on health and disease in later life through programming of immune and metabolic pathways. We collected maternal faeces, placenta, amniotic fluid, colostrum, meconium and infant faeces samples from 15 mother-infant pairs in an effort to rigorously investigate prenatal and neonatal microbial transfer and gut colonisation. To ensure sterile sampling, only deliveries at full term by elective caesarean section were studied. Microbiota composition and activity assessment by conventional bacterial culture, 16S rRNA gene pyrosequencing, quantitative PCR, and denaturing gradient gel electrophoresis revealed that the placenta and amniotic fluid harbour a distinct microbiota characterised by low richness, low diversity and the predominance of Proteobacteria. Shared features between the microbiota detected in the placenta and amniotic fluid and in infant meconium suggest microbial transfer at the foeto-maternal interface. At the age of 3-4 days, the infant gut microbiota composition begins to resemble that detected in colostrum. Based on these data, we propose that the stepwise microbial gut colonisation process may be initiated already prenatally by a distinct microbiota in the placenta and amniotic fluid. The link between the mother and the offspring is continued after birth by microbes present in breast milk.

PMID:
27001291
PMCID:
PMC4802384
DOI:
10.1038/srep23129
[Indexed for MEDLINE]
Free PMC Article

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