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Biochim Biophys Acta. 2016 May;1859(5):731-43. doi: 10.1016/j.bbagrm.2016.03.008. Epub 2016 Mar 18.

Rpb1 foot mutations demonstrate a major role of Rpb4 in mRNA stability during stress situations in yeast.

Author information

1
Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071 Jaén, Spain.
2
Departamento de Genética, Facultad de Biológicas, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain; ERI Biotecmed, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain.
3
European Molecular Biology Laboratories (EMBL), Genome Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
4
ERI Biotecmed, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain; Departamento de Bioquímica y Biología Molecular, Facultad de Biológicas, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain.
5
ERI Biotecmed, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain; Departamento de Bioquímica y Biología Molecular, Facultad de Biológicas, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain. Electronic address: jose.e.perez@uv.es.
6
Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071 Jaén, Spain. Electronic address: fngomez@ujaen.es.

Abstract

The RPB1 mutants in the foot region of RNA polymerase II affect the assembly of the complex by altering the correct association of both the Rpb6 and the Rpb4/7 dimer. Assembly defects alter both transcriptional activity as well as the amount of enzyme associated with genes. Here, we show that the global transcriptional analysis of foot mutants reveals the activation of an environmental stress response (ESR), which occurs at a permissive temperature under optimal growth conditions. Our data indicate that the ESR that occurs in foot mutants depends mostly on a global post-transcriptional regulation mechanism which, in turn, depends on Rpb4-mRNA imprinting. Under optimal growth conditions, we propose that Rpb4 serves as a key to globally modulate mRNA stability as well as to coordinate transcription and decay. Overall, our results imply that post-transcriptional regulation plays a major role in controlling the ESR at both the transcription and mRNA decay levels.

KEYWORDS:

Assembly; Rpb4; Stability; Stress; mRNA

PMID:
27001033
DOI:
10.1016/j.bbagrm.2016.03.008
[Indexed for MEDLINE]

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