Format

Send to

Choose Destination
J Dent Res. 2016 Jul;95(8):882-8. doi: 10.1177/0022034516637579. Epub 2016 Mar 18.

Integration-Free Reprogramming of Lamina Propria Progenitor Cells.

Author information

1
Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, Wales, UK howardjonesRA1@cf.ac.uk.
2
Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, Wales, UK.
3
School of Biosciences, College of Biomedical and Life Sciences, Cardiff University, Cardiff, Wales, UK.

Abstract

Producing induced pluripotent stem cells (iPSCs) from human tissue for use in personalized medicine strategies or therapeutic testing is at the forefront of medicine. Therefore, identifying a source of cells to reprogram that is easily accessible via a simple noninvasive procedure is of great clinical importance. Reprogramming these cells to iPSCs through nonintegrating methods for genetic manipulation is paramount for regenerative purposes. Here, we demonstrate reprogramming of oral mucosal lamina propria progenitor cells from patients undergoing routine dental treatment. Reprogramming was performed utilizing nonintegrating plasmids containing all 6 pluripotency genes (OCT4, SOX2, KLF4, NANOG, LIN28, and cMYC). Resulting iPSCs lacked genetic integration of the vector genes and had the ability to differentiate down mesoderm, ectoderm, and endoderm lineages, demonstrating pluripotency. In conclusion, oral mucosal lamina propria progenitor cells represent a source of cells that can be obtained with minimal invasion, as they can be taken concurrently with routine treatments. The resulting integration-free iPSCs therefore have great potential for use in personalized medicine strategies.

KEYWORDS:

IPS cells; hiPSCs; human induced pluripotent stem cells; oral mucosa; regenerative medicine; stem cells

PMID:
26994108
DOI:
10.1177/0022034516637579
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center