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Talanta. 2016 May 15;152:269-76. doi: 10.1016/j.talanta.2016.02.017. Epub 2016 Feb 6.

Improved sensitivity of lateral flow assay using paper-based sample concentration technique.

Author information

1
School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, PR China; Key Laboratory for Space Bioscience and Biotechnology, Northwestern Polytechnical University, Xi'an 710072, PR China; The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China.
2
School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, PR China; Key Laboratory for Space Bioscience and Biotechnology, Northwestern Polytechnical University, Xi'an 710072, PR China. Electronic address: kittyyh@nwpu.edu.cn.
3
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China; Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
4
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China.
5
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
6
School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, PR China; Key Laboratory for Space Bioscience and Biotechnology, Northwestern Polytechnical University, Xi'an 710072, PR China.
7
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China. Electronic address: fengxu@mail.xjtu.edu.cn.

Abstract

Lateral flow assays (LFAs) hold great promise for point-of-care testing, especially in resource-poor settings. However, the poor sensitivity of LFAs limits their widespread applications. To address this, we developed a novel device by integrating dialysis-based concentration method into LFAs. The device successfully achieved 10-fold signal enhancement in Human Immunodeficiency Virus (HIV) nucleic acid detection with a detection limit of 0.1 nM and 4-fold signal enhancement in myoglobin (MYO) detection with a detection limit of 1.56 ng/mL in less than 25 min. This simple, low-cost and portable integrated device holds great potential for highly sensitive detection of various target analytes for medical diagnostics, food safety analysis and environmental monitoring.

KEYWORDS:

Dialysis-based concentration; HIV; Lateral flow assay; MYO; Paper-based device

PMID:
26992520
DOI:
10.1016/j.talanta.2016.02.017
[Indexed for MEDLINE]

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