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PeerJ. 2016 Mar 14;4:e1792. doi: 10.7717/peerj.1792. eCollection 2016.

The effect of ribosomal protein S15a in lung adenocarcinoma.

Author information

1
Department of Thoracic Surgery, The Second Hospital of Jilin University , Changchun, Jilin , China.
2
Jilin provincial key laboratory on molecular and chemical genetic, The Second Hospital of Jilin University , Changchun , China.

Abstract

BACKGROUND:

RPS15A (Ribosomal Protein S15A) promotes mRNA/ribosome interactions in translation. It is critical for the process of eukaryotic protein biosynthesis. Recently, aberrantly expressed RPS15A was found in the hepatitis virus and in malignant tumors. However, the role of RPS15A has not been fully revealed on the development of lung cancer.

METHOD:

In this study, a Tissue Microarray (TMA) of primary lung adenocarcinoma tissue specimens was carried out. Furthermore, to further investigate the function of RPS15A in lung cancer, RPS15A-specific short hairpin RNA (shRNA) expressing lentivirus (Lv-shRPS15A) was constructed and used to infect H1299 and A549 cells.

RESULT:

Our data showed that RPS15A expression was increased in tumor tissues. Furthermore, the knockdown of RSP15A inhibited cancer cell growth and induced apoptosis in the cancer cells. Gene expression profile microarray also revealed that the P53 signaling pathway was activated in Lv-shRPS15A-infected cancer cells.

CONCLUSION:

Taken together, our results demonstrate that RPS15A is a novel oncogene in non-small cell lung cancer and may be a potential therapeutic target in lung cancer.

KEYWORDS:

Lung adenocarcinoma; P53 pathway; RPS15A; Tissue microarray; shRNA

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