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Cancer Lett. 2016 Jun 1;375(2):284-292. doi: 10.1016/j.canlet.2016.03.016. Epub 2016 Mar 14.

B4GALT3 up-regulation by miR-27a contributes to the oncogenic activity in human cervical cancer cells.

Author information

1
Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
2
Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China. Electronic address: htang2002@yahoo.com.

Abstract

β-1,4-Galactosyltransferase III (B4GALT3) is an enzyme responsible for the generation of poly-N-acetyllactosamine and is involved in tumorigenesis. However, B4GALT3-dysregulation and its role in cervical cancer cells are unknown. Herein, we found that B4GALT3 was upregulated in cervical cancer tissues compared to adjacent non-tumor tissues. B4GALT3-overexpression promoted, whereas B4GALT3-knockdown suppressed the cellular migration, invasion and EMT of HeLa and C33A cervical cancer cells. To explore the mechanism of dysregulation, B4GALT3 was predicted to be a target of miR-27a. EGFP and pGL3-promoter reporter assay showed miR-27a binds to B4GALT3 3'UTR region but enhanced its expression. RT-qPCR showed miR-27a was also upregulated and presented positive correlation with B4GALT3-expression in cervical cancer tissues. miR-27a-overexpression promoted, but blocking-miR-27a repressed these malignancies in HeLa and C33A cells. Furthermore, shR-B4GALT3 counteracted the promotion of malignancies induced by miR-27a, suggesting miR-27a upregulates B4GALT3 to enhance tumorigenic activities. In addition, we found that B4GALT3 significantly enhances β1-integrin stability, thus mediating promotion of B4GALT3 on malignancy in cervical cancer cells. Altogether, our findings evidenced that B4GALT3 upregulated by miR-27a contributes to the tumorigenic activities by β1-integrin pathway and might provide potential biomarkers for cervical cancer.

KEYWORDS:

B4GALT3; Cervical cancer cells; EMT; miR-27a; β1-integrin

PMID:
26987623
DOI:
10.1016/j.canlet.2016.03.016
[Indexed for MEDLINE]

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