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Sci Rep. 2016 Mar 17;6:23165. doi: 10.1038/srep23165.

A new hand-held microfluidic cytometer for evaluating irradiation damage by analysis of the damaged cells distribution.

Author information

1
College of Information and Science Technology, Dalian Maritime University, Dalian, 116026, China.
2
College of Marine Engineering, Dalian Maritime University, Dalian, 116026, China.
3
College of Environmental Science and Engineering, Dalian Maritime University, Dalian, 116026, China.
4
Department of Mechanical &Mechatronics Engineering, University of Waterloo, Waterloo, ON N2L3G1, Canada.

Abstract

Space radiation brings uneven damages to cells. The detection of the distribution of cell damage plays a very important role in radiation medicine and the related research. In this paper, a new hand-held microfluidic flow cytometer was developed to evaluate the degree of radiation damage of cells. The device we propose overcomes the shortcomings (e.g., large volume and high cost) of commercial flow cytometers and can evaluate the radiation damage of cells accurately and quickly with potential for onsite applications. The distribution of radiation-damaged cells is analyzed by a simultaneous detection of immunofluorescence intensity of γ-H2AX and resistance pulse sensor (RPS) signal. The γ-H2AX fluorescence intensity provides information of the degree of radiation damage in cells. The ratio of the number of cells with γ-H2AX fluorescence signals to the total numbers of cells detected by RPS indicates the percentage of the cells that are damaged by radiation. The comparison experiment between the developed hand-held microfluidic flow cytometer and a commercial confocal microscope indicates a consistent and comparable detection performance.

PMID:
26983800
PMCID:
PMC4794725
DOI:
10.1038/srep23165
[Indexed for MEDLINE]
Free PMC Article

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