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J Recept Signal Transduct Res. 2016 Dec;36(6):601-616. Epub 2016 Mar 16.

Probing binding mechanism of interleukin-6 and olokizumab: in silico design of potential lead antibodies for autoimmune and inflammatory diseases.

Author information

1
a Biomedical Informatics Centre, National Institute of Pathology - ICMR , New Delhi , India and.
2
b Bioinformatics Infrastructure Facility, University of Kalyani , Kalyani, West Bengal , India.

Abstract

Computer-aided antibody engineering has been successful in the design of new biologics for disease diagnosis and therapeutic interventions. Interleukin-6 (IL-6), a well-recognized drug target for various autoimmune and inflammatory diseases such as rheumatoid arthritis, multiple sclerosis, and psoriasis, was investigated in silico to design potential lead antibodies. Here, crystal structure of IL-6 along with monoclonal antibody olokizumab was explored to predict antigen-antibody (Ag - Ab)-interacting residues using DiscoTope, Paratome, and PyMOL. Tyr56, Tyr103 in heavy chain and Gly30, Ile31 in light chain of olokizumab were mutated with residues Ser, Thr, Tyr, Trp, and Phe. A set of 899 mutant macromolecules were designed, and binding affinity of these macromolecules to IL-6 was evaluated through Ag - Ab docking (ZDOCK, ClusPro, and Rosetta server), binding free-energy calculations using Molecular Mechanics/Poisson Boltzman Surface Area (MM/PBSA) method, and interaction energy estimation. In comparison to olokizumab, eight newly designed theoretical antibodies demonstrated better result in all assessments. Therefore, these newly designed macromolecules were proposed as potential lead antibodies to serve as a therapeutics option for IL-6-mediated diseases.

KEYWORDS:

Interleukin-6; antigen–antibody docking; autoimmune and inflammatory diseases; computer-aided antibody engineering; olokizumab

PMID:
26982101
DOI:
10.3109/10799893.2016.1147584
[Indexed for MEDLINE]

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