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Nat Commun. 2016 Mar 16;7:11036. doi: 10.1038/ncomms11036.

Cellular forces and matrix assembly coordinate fibrous tissue repair.

Author information

1
Institute of Robotics and Intelligent Systems, Eidgenössische Technische Hochschule Zürich, 8092 Zurich, Switzerland.
2
Department of Biomedical Engineering, Boston University, 36 Cummington Mall, Boston, Massachusetts 02215, USA.
3
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA.
4
Laboratory for Urologic Tissue Engineering and Stem Cell Therapy, Department of Urology, University Hospital, Zurich 8091, Switzerland.

Abstract

Planar in vitro models have been invaluable tools to identify the mechanical basis of wound closure. Although these models may recapitulate closure dynamics of epithelial cell sheets, they fail to capture how a wounded fibrous tissue rebuilds its 3D architecture. Here we develop a 3D biomimetic model for soft tissue repair and demonstrate that fibroblasts ensconced in a collagen matrix rapidly close microsurgically induced defects within 24 h. Traction force microscopy and time-lapse imaging reveal that closure of gaps begins with contractility-mediated whole-tissue deformations. Subsequently, tangentially migrating fibroblasts along the wound edge tow and assemble a progressively thickening fibronectin template inside the gap that provide the substrate for cells to complete closure. Unlike previously reported mechanisms based on lamellipodial protrusions and purse-string contraction, our data reveal a mode of stromal closure in which coordination of tissue-scale deformations, matrix assembly and cell migration act together to restore 3D tissue architecture.

PMID:
26980715
PMCID:
PMC4799373
DOI:
10.1038/ncomms11036
[Indexed for MEDLINE]
Free PMC Article

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