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Cell Adh Migr. 2016 Jul 3;10(4):331-41. doi: 10.1080/19336918.2015.1129482. Epub 2016 Mar 16.

Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching.

Author information

1
a Faculty of Science , Yamaguchi University , Yamaguchi , Japan.
2
b School of Information Science and Technology , Aichi Prefectural University , Aichi , Japan.
3
c Graduate School of Biological Sciences , Nara Institute of Science and Technology , Nara , Japan.

Abstract

To investigate the relationship between mechanical stimuli from substrata and related cell functions, one of the most useful techniques is the application of mechanical stimuli via periodic stretching of elastic substrata. In response to this stimulus, Dictyostelium discoideum cells migrate in a direction perpendicular to the stretching direction. The origins of directional migration, higher migration velocity in the direction perpendicular to the stretching direction or the higher probability of a switch of migration direction to perpendicular to the stretching direction, however, remain unknown. In this study, we applied periodic stretching stimuli to neutrophil-like differentiated HL-60 cells, which migrate perpendicular to the direction of stretch. Detailed analysis of the trajectories of HL-60 cells and Dictyostelium cells obtained in a previous study revealed that the higher probability of a switch of migration direction to that perpendicular to the direction of stretching was the main cause of such directional migration. This directional migration appears to be a strategy adopted by fast-crawling cells in which they do not migrate faster in the direction they want to go, but migrate to avoid a direction they do not want to go.

KEYWORDS:

Dictyostelium; HL-60; amoeboid movement; cell migration; mechanosensing; neutrophil; trajectory analysis

PMID:
26980079
PMCID:
PMC4986713
DOI:
10.1080/19336918.2015.1129482
[Indexed for MEDLINE]
Free PMC Article

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