Format

Send to

Choose Destination
Bioanalysis. 2016 Apr;8(7):625-32. doi: 10.4155/bio-2015-0034. Epub 2016 Mar 15.

New analytical application of antibody-based biosensor in estimation of thyroid-stimulating hormone in serum.

Author information

1
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia.
2
Department of Biochemistry, College of Science, King Saud University, PO Box 22452, Riyadh 11451, Saudi Arabia.
3
Department of Genetics, King Faisal Specialist Hospital & Research Centre, Riyadh 11211, Saudi Arabia.

Abstract

BACKGROUND:

Conventionally, ELISA is used to measure thyroid-stimulating hormone (TSH) for diagnosis of thyroid disease. In this study, an immunosensor-based, kinetic-exclusion analysis (KinExA) was used for TSH estimation.

METHODOLOGY:

A PMMA microbead column coated with TSH antigen is formed inside the flow cell. Samples consisting of mouse anti-TSH monoclonal antibody and TSH antigen complex in solution are passed over the beads and the unbound anti-TSH antibody is captured by the TSH-coated beads, followed by passing fluorescent-labeled antibody over the beads to generate signals for analysis. The limit of detection for the assay was 0.4 mIU l(-1) and the precision was acceptable.

CONCLUSION:

The developed sensor was advantageous due to the automated nature and its convenience, without compromising the sensitivity for estimation of TSH.

KEYWORDS:

ELISA; TSH; biosensor; thyroid disease

PMID:
26978548
DOI:
10.4155/bio-2015-0034
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center