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J Gen Microbiol. 1989 Sep;135(9):2561-7.

Transformation in restriction-deficient Salmonella typhimurium LT2.

Author information

1
Department of Microbiology, School of Medicine, Loma Linda University, CA 92350.

Abstract

Stable restriction-deficient, modification-proficient galE (JR501) and F'galE+ (JR502) strains of Salmonella typhimurium were constructed and the effects of restriction on transformation by plasmid pBR322 were tested. Several factors which affect transformation efficiency were systematically examined to determine optimum transformation conditions and a simplified method is presented.

PMID:
2697751
DOI:
10.1099/00221287-135-9-2561
[Indexed for MEDLINE]

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