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Biomed Opt Express. 2016 Jan 6;7(2):324-34. doi: 10.1364/BOE.7.000324. eCollection 2016 Feb 1.

Deep in vivo two-photon microscopy with a low cost custom built mode-locked 1060 nm fiber laser.

Author information

1
Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, Texas 78712, USA.

Abstract

Here we demonstrate that a mode-locked ytterbium fiber laser for two-photon fluorescence microscopy can be built for $13,000. The laser emits at a wavelength of 1060 nm with a usable average power of 1 W at a repetition rate of 40 MHz and a compressed pulse width of 81 fs at the sample. The laser is used to obtain deep in vivo two-color images of layer-V pyramidal neurons expressing YFP and vasculature labelled with Texas Red at depths up to 900 µm. The sub-1 µm features of dendritic spines can be resolved at a 200 µm depth.

KEYWORDS:

(140.4050) Mode-locked lasers; (170.3880) Medical and biological imaging; (190.4180) Multiphoton processes

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