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Anticancer Res. 2016 Mar;36(3):1135-42.

The Association Between WAVE1 and -3 and the ARP2/3 Complex in PC 3 Cells.

Author information

1
Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Heath Park, Cardiff, U.K.
2
Institute of Cancer and Genetics, Cardiff University School of Medicine, Heath Park, Cardiff, U.K.
3
Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Heath Park, Cardiff, U.K. jiangw@cardiff.ac.uk.

Abstract

BACKGROUND:

Actin polymerisation is stimulated by the actin-related protein (ARP) 2/3 complex and drives cell migration. This complex is activated by Wiskott-Aldrich syndrome protein family (WASP) verprolin homologous protein (WAVE) proteins. WAVE1 and -3 have been implicated in the aggressiveness of metastatic prostate cancer cells.

MATERIALS AND METHODS:

Cell growth, motility and invasion were analyzed in WAVE1- and WAVE3-knockdown PC-3 cells along with the ARP2/3 inhibitor, CK-0944636. Confocal microscopy was adopted to examine protein co-localisation. Immunoprecipitation approaches were used to determine protein tyrosine phosphorylation.

RESULTS:

Cell growth suppression was observed with WAVE3 knockdown and ARP2/3 inhibition. Reduced cell invasion effects observed with WAVE1 knockdown appeared to be rescued by ARP2/3 inhibition. WAVE1 and WAVE3 and ARP2 co-localisation was lost in PC-3 WAVE-knockdown cells, while increased ARP2 tyrosine phosphorylation was observed with WAVE3 knockdown.

CONCLUSION:

These results implicate a contributory role of WAVE1 and -3 to the metastatic phenotype of PC-3 cells through their interaction with the ARP2/3 complex.

KEYWORDS:

ARP2/3 complex; WAVE1; WAVE3; prostate cancer

PMID:
26977009
[Indexed for MEDLINE]

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