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J Pediatr Surg. 2016 Jul;51(7):1192-6. doi: 10.1016/j.jpedsurg.2016.01.009. Epub 2016 Feb 3.

Protective effect of pterostilbene on testicular ischemia/reperfusion injury in rats.

Author information

1
The Institute for the 3Rs & Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
2
Department of Animal Science, Shingu College University, 2 Geumgwang-dong, Jungwon-gu, Seongnam-si, Gyeonggi-do 462-743, Republic of Korea.
3
Department of Pet Science, Seojeong College University, Yongam-ri, Eunhyeon-m yeon, Yangju-si, Gyeonggi-do 482-777, Republic of Korea.
4
Laboratory Animal Research Center, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
5
The Institute for the 3Rs & Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea. Electronic address: labvet@konkuk.ac.kr.

Abstract

PURPOSE:

To evaluate protective effect of pterostilbene against testicular ischemia/reperfusion (I/R) injury, which results in increased formation of oxidative stress, leading to testicular apoptosis and impaired spermatogenesis.

METHODS:

Thirty two pubertal male Sprague-Dawley rats weighing 180-220g were selected and randomly divided into the following four groups: group A (normal control group), group B (sham-operated group), group C (induced I/R injury group), group D (induced I/R injury group receiving pterostilbene treatment). Johnsen's scores and mean seminiferous tubule diameters were evaluated for histopathologic assessment; germinal cell apoptosis was evaluated by the transferase dUTP nick end labeling (TUNEL) assay and immunohistochemistry for caspases. Malondialdehyde (MDA) levels were assessed as an indicator of oxidative stress and total antioxidant capacity (TAC) was measured.

RESULTS:

Germ cell apoptosis and MDA level significantly increased whereas TAC significantly decreased in group C; moreover, abnormal morphology and impaired spermatogenesis were observed in group C. In contrast, treatment with pterostilbene inhibited lipid peroxidation and apoptosis induced by ROS and restored the antioxidant capacity in group D.

CONCLUSIONS:

These results show that treatment with pterostilbene may be a promising therapy for testicular I/R injury.

KEYWORDS:

Antioxidant; Apoptosis; Caspases; Testicular torsion

PMID:
26976774
DOI:
10.1016/j.jpedsurg.2016.01.009
[Indexed for MEDLINE]

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